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alexa fluor 488 affinipure donkey anti mouse igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 488 affinipure donkey anti mouse igg

    Alexa Fluor 488 Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+mouse/Alexa+Fluor+488+AffiniPure+Donkey+Anti-Mouse+IgG/pmc13657110-418-13-23
    Average 96 stars, based on 1824 article reviews
    alexa fluor 488 affinipure donkey anti mouse igg - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models"

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    Journal: iScience

    doi: 10.1016/j.isci.2026.117673


    Figure Legend Snippet:

    Techniques Used: Recombinant, Mutagenesis, Software, Analysis, Silicon

    Related Articles

    Neuroscience:

    Article Title: TNF-α modulates Hippo and Wnt/β-catenin signaling pathways in retinal Müller cells in experimental glaucoma
    Article Snippet: .. Visual Neuroscience 2026, 43: e023 ImmunoResearch Labs), Alexa Fluor 488-conjugated donkey antirabbit (1:500, #711-545-152, Jackson ImmunoResearch Labs), Cy3conjugated donkey anti- mouse (1:500, #715-165-151, Jackson ImmunoResearch Labs), and Alexa Fluor 594-conjugated donkey anti-rabbit (1:500, #711-585-152, Jackson ImmunoResearch Labs). ..

    Immunostaining:

    Article Title: Inhibition of T cell infiltration and soluble TNF signaling is neuroprotective in the alpha-synuclein overexpressing mouse model of Parkinson's disease.
    Article Snippet: .. For colorimetric immunostaining, sections were incubated with the biotinylated secondary antibodies goat anti-rabbit (1:500; Jackson ImmunoResearch, Ely, UK) and donkey anti-mouse (1:500; Jackson ImmunoResearch) in blocking solution for 2 h at RT, followed by incubation with peroxidase-conjugated avidin in PBS (1:5000; Sigma-Aldrich) for 90min at RT. .. After washing with PBS, all sections were simultaneously incubated in the same batch of 0.05% diaminobenzidine (Sigma-Aldrich), 0.03%H2O2, and Trizma-HCl buffer (pH 7.6) for the same time.

    Incubation:

    Article Title: Inhibition of T cell infiltration and soluble TNF signaling is neuroprotective in the alpha-synuclein overexpressing mouse model of Parkinson's disease.
    Article Snippet: .. For colorimetric immunostaining, sections were incubated with the biotinylated secondary antibodies goat anti-rabbit (1:500; Jackson ImmunoResearch, Ely, UK) and donkey anti-mouse (1:500; Jackson ImmunoResearch) in blocking solution for 2 h at RT, followed by incubation with peroxidase-conjugated avidin in PBS (1:5000; Sigma-Aldrich) for 90min at RT. .. After washing with PBS, all sections were simultaneously incubated in the same batch of 0.05% diaminobenzidine (Sigma-Aldrich), 0.03%H2O2, and Trizma-HCl buffer (pH 7.6) for the same time.

    Article Title: CX3CL1/CX3CR1 involved modulation of microglial activation and eosinophilic meningoencephalitis in angiostrongyliasis
    Article Snippet: Membranes were blocked with 3 % BSA in PBS for 1 h at room temperature, washed with PBST (0.1 % Tween-20 PBS), and probed overnight at 4 °C with the following primary antibodies (Iba-1 (Novus, Cat: NB100–1028, USA); CX3CR1 (ABclone, USA); CX3CL1 (ABclone, Cat: A2890, USA); p-tau (S396) (abcam, Cambridge, Cat: A14198, MA, USA); tau (abcam, Cambridge, Cat: ab254256, MA, USA); NeuN (ABclone, Cat: A0951, USA); Nrf2 (ABclone, Cat: A11159, USA); JNK (ABclone, Cat: ab179461, USA); HO-1 (Sanat cruz, Cat: sc-1797,USA) diluted 1:1000; β-Actin, diluted 1:10000 (sigma, Cat: sc-47,778, USA)) at 4 °C overnight. .. After PBST washes, membranes were incubated with HRP-conjugated secondary antibody (Goat anti-Rabbit (Code: 111–035-144, Lot: 115641); Donkey anti-mouse (Code: 715–036-150, Lot: 156476), Jackson ImmunoResearch) for 1 h at 37 °C. .. Protein bands were visualized using enhanced chemiluminescence on an iBright CL750 system (Thermo Fisher Scientific).

    Article Title: Propafenone-mediated gap junctional uncoupling results from aberrant connexin-43 trafficking.
    Article Snippet: Primary antibodies used were Cx43 (1:250, BD Biosciences) and zonula occludens (ZO-1, 1:150, Invitrogen, Rockford, IL, USA, cat. no. 61-7300). .. After washing the cells, coverslips were incubated with secondary antibodies: donkey anti-mouse (Green, 1:100; Jackson ImmunoResearch, West Grove, PA, USA, cat. no. 715-095-151) and donkey anti-rabbit (Alexa Red, 1:350; Jackson ImmunoResearch, cat. no. 711-585-152). .. Cell nuclei were stained with 4’, 6-diamindino-2-phenylindole (DAPI; Molecular Probes, Leiden, Netherlands).

    Blocking Assay:

    Article Title: Inhibition of T cell infiltration and soluble TNF signaling is neuroprotective in the alpha-synuclein overexpressing mouse model of Parkinson's disease.
    Article Snippet: .. For colorimetric immunostaining, sections were incubated with the biotinylated secondary antibodies goat anti-rabbit (1:500; Jackson ImmunoResearch, Ely, UK) and donkey anti-mouse (1:500; Jackson ImmunoResearch) in blocking solution for 2 h at RT, followed by incubation with peroxidase-conjugated avidin in PBS (1:5000; Sigma-Aldrich) for 90min at RT. .. After washing with PBS, all sections were simultaneously incubated in the same batch of 0.05% diaminobenzidine (Sigma-Aldrich), 0.03%H2O2, and Trizma-HCl buffer (pH 7.6) for the same time.

    Avidin-Biotin Assay:

    Article Title: Inhibition of T cell infiltration and soluble TNF signaling is neuroprotective in the alpha-synuclein overexpressing mouse model of Parkinson's disease.
    Article Snippet: .. For colorimetric immunostaining, sections were incubated with the biotinylated secondary antibodies goat anti-rabbit (1:500; Jackson ImmunoResearch, Ely, UK) and donkey anti-mouse (1:500; Jackson ImmunoResearch) in blocking solution for 2 h at RT, followed by incubation with peroxidase-conjugated avidin in PBS (1:5000; Sigma-Aldrich) for 90min at RT. .. After washing with PBS, all sections were simultaneously incubated in the same batch of 0.05% diaminobenzidine (Sigma-Aldrich), 0.03%H2O2, and Trizma-HCl buffer (pH 7.6) for the same time.

    other:

    Article Title: Precise excision of expanded GGC repeats in NOTCH2NLC via CRISPR/Cas9 for treating neuronal intranuclear inclusion disease.
    Article Snippet: HRP-conjugated secondary antibodies (1:10000) were donkey anti-rabbit and donkey anti-mouse from Jackson ImmunoResearch.

    Western Blot:

    Article Title: Establishment of γ-secretase-deficient goblet-like cells: A novel in vitro platform to dissect regulatory mechanisms of mucus production in the intestinal epithelium.
    Article Snippet: Intestinal goblet cells play a crucial role in producing mucus that forms the protective mucosal barrier.. However, the complex in vivo interactions among microbial, dietary, and immune factors make it challenging to dissect the intrinsic regulatory mechanisms underlying goblet cell mucus production.. In this study, we established a novel HT-29–derived cell line lacking Presenilin-1 and Nicastrin, two core components of γ-secretase, a membraneassociated protease complex that cleaves multiple transmembrane proteins, including Notch receptors.

    Immunofluorescence:

    Article Title: Establishment of γ-secretase-deficient goblet-like cells: A novel in vitro platform to dissect regulatory mechanisms of mucus production in the intestinal epithelium.
    Article Snippet: Intestinal goblet cells play a crucial role in producing mucus that forms the protective mucosal barrier.. However, the complex in vivo interactions among microbial, dietary, and immune factors make it challenging to dissect the intrinsic regulatory mechanisms underlying goblet cell mucus production.. In this study, we established a novel HT-29–derived cell line lacking Presenilin-1 and Nicastrin, two core components of γ-secretase, a membraneassociated protease complex that cleaves multiple transmembrane proteins, including Notch receptors.

    Staining:

    Article Title: Establishment of γ-secretase-deficient goblet-like cells: A novel in vitro platform to dissect regulatory mechanisms of mucus production in the intestinal epithelium.
    Article Snippet: Intestinal goblet cells play a crucial role in producing mucus that forms the protective mucosal barrier.. However, the complex in vivo interactions among microbial, dietary, and immune factors make it challenging to dissect the intrinsic regulatory mechanisms underlying goblet cell mucus production.. In this study, we established a novel HT-29–derived cell line lacking Presenilin-1 and Nicastrin, two core components of γ-secretase, a membraneassociated protease complex that cleaves multiple transmembrane proteins, including Notch receptors.



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    Image Search Results


    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: Sections were then washed in 1 x PBS four times and incubated in Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L, Jackson ImmunoResearch, cat no: 715–545-150, West Grove, Pennsylvania), 1:200 dilution in 1% normal donkey serum in 0.5% Triton X-100 overnight at 4°C.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon